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wi 38 va13 subline 2ra cells  (ATCC)


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    Structured Review

    ATCC wi 38 va13 subline 2ra cells
    (A) Immunofluorescence of DKC1[A353V] fibroblasts (Fib p5) and iPSCs clones 1, 2, 3, and 3-tf at indicated passages (p). PML bodies were stained with an anti-PML antibody (red), and telomeres were labeled with FITC-conjugated PNA probe (green). Interphase nuclei were counterstained with DAPI (blue). Colocalization of PML bodies and telomeres was detected only in <t>VA13</t> cells (positive control). (B) Dot blot of C-circle assay performed on genomic DNA from DKC1[A353V] fibroblasts (Fib p6) and iPSCs clones 1, 1-tf, 2, and 3 at indicated passages (p), as well as iPSCs from control (Ctrl iPSCs clone 26). Genomic DNA from the ALT-positive cell lines U2-OS and Saos-2 was set as positive control.
    Wi 38 Va13 Subline 2ra Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wi+38+va13+subline+2ra+cells/WI-38+VA-13+subline+2RA/pmc08376098-141-1-9
    Average 94 stars, based on 102 article reviews
    wi 38 va13 subline 2ra cells - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Telomere dynamics and hematopoietic differentiation of human DKC1 -mutant induced pluripotent stem cells"

    Article Title: Telomere dynamics and hematopoietic differentiation of human DKC1 -mutant induced pluripotent stem cells

    Journal: Stem cell research

    doi: 10.1016/j.scr.2019.101540

    (A) Immunofluorescence of DKC1[A353V] fibroblasts (Fib p5) and iPSCs clones 1, 2, 3, and 3-tf at indicated passages (p). PML bodies were stained with an anti-PML antibody (red), and telomeres were labeled with FITC-conjugated PNA probe (green). Interphase nuclei were counterstained with DAPI (blue). Colocalization of PML bodies and telomeres was detected only in VA13 cells (positive control). (B) Dot blot of C-circle assay performed on genomic DNA from DKC1[A353V] fibroblasts (Fib p6) and iPSCs clones 1, 1-tf, 2, and 3 at indicated passages (p), as well as iPSCs from control (Ctrl iPSCs clone 26). Genomic DNA from the ALT-positive cell lines U2-OS and Saos-2 was set as positive control.
    Figure Legend Snippet: (A) Immunofluorescence of DKC1[A353V] fibroblasts (Fib p5) and iPSCs clones 1, 2, 3, and 3-tf at indicated passages (p). PML bodies were stained with an anti-PML antibody (red), and telomeres were labeled with FITC-conjugated PNA probe (green). Interphase nuclei were counterstained with DAPI (blue). Colocalization of PML bodies and telomeres was detected only in VA13 cells (positive control). (B) Dot blot of C-circle assay performed on genomic DNA from DKC1[A353V] fibroblasts (Fib p6) and iPSCs clones 1, 1-tf, 2, and 3 at indicated passages (p), as well as iPSCs from control (Ctrl iPSCs clone 26). Genomic DNA from the ALT-positive cell lines U2-OS and Saos-2 was set as positive control.

    Techniques Used: Immunofluorescence, Clone Assay, Staining, Labeling, Positive Control, Dot Blot, Control

    Related Articles

    Positive Control:

    Article Title: Telomere dynamics and hematopoietic differentiation of human DKC1 -mutant induced pluripotent stem cells
    Article Snippet: Images were acquired in the Applied Spectral Imaging software (Applied Spectral Imaging Ltd). .. Telomerase-deficient WI 38 VA13 subline 2RA cells (VA13, from ATCC) were used as the positive control. ..

    Article Title: Telomere dynamics and hematopoietic differentiation of human DKC1-mutant induced pluripotent stem cells.
    Article Snippet: Images were acquired in the Applied Spectral Imaging software (Applied Spectral Imaging Ltd). .. Telomerase-deficient WI 38 VA13 subline 2RA cells (VA13, from ATCC) were used as the positive control. ..

    Incubation:

    Article Title: A mutation in the H/ACA box of telomerase RNA component gene ( TERC ) in a young patient with myelodysplastic syndrome
    Article Snippet: .. Telomerase-deficient WI 38 VA13 subline 2RA cells (VA13; ATCC, Manassas, VA, USA) were plated on six-well culture plates (4 x 10 5 cells/ well) and incubated for 24 hours. .. Either pcDNA3-wtTERC (2 μg) or pcDNA3- mutTERC (2 μg) was cotransfected with pcDNA3-Flag-TERT (2 μg) into the VA13 cells using X-tremeGENE HP DNA Transfection Reagent (12 μl, Roche Applied Science).

    Article Title: A mutation in the H/ACA box of telomerase RNA component gene (TERC) in a young patient with myelodysplastic syndrome.
    Article Snippet: .. Telomerase-deficient WI 38 VA13 subline 2RA cells (VA13; ATCC, Manassas, VA, USA) were plated on six-well culture plates (4 x 105 cells/ well) and incubated for 24 hours. .. Either pcDNA3-wtTERC (2 μg) or pcDNA3- mutTERC (2 μg) was cotransfected with pcDNA3-Flag-TERT (2 μg) into the VA13 cells using X-tremeGENE HP DNA Transfection Reagent (12 μl, Roche Applied Science).



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    (A) Immunofluorescence of DKC1[A353V] fibroblasts (Fib p5) and iPSCs clones 1, 2, 3, and 3-tf at indicated passages (p). PML bodies were stained with an anti-PML antibody (red), and telomeres were labeled with FITC-conjugated PNA probe (green). Interphase nuclei were counterstained with DAPI (blue). Colocalization of PML bodies and telomeres was detected only in <t>VA13</t> cells (positive control). (B) Dot blot of C-circle assay performed on genomic DNA from DKC1[A353V] fibroblasts (Fib p6) and iPSCs clones 1, 1-tf, 2, and 3 at indicated passages (p), as well as iPSCs from control (Ctrl iPSCs clone 26). Genomic DNA from the ALT-positive cell lines U2-OS and Saos-2 was set as positive control.
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    (A) Immunofluorescence of DKC1[A353V] fibroblasts (Fib p5) and iPSCs clones 1, 2, 3, and 3-tf at indicated passages (p). PML bodies were stained with an anti-PML antibody (red), and telomeres were labeled with FITC-conjugated PNA probe (green). Interphase nuclei were counterstained with DAPI (blue). Colocalization of PML bodies and telomeres was detected only in <t>VA13</t> cells (positive control). (B) Dot blot of C-circle assay performed on genomic DNA from DKC1[A353V] fibroblasts (Fib p6) and iPSCs clones 1, 1-tf, 2, and 3 at indicated passages (p), as well as iPSCs from control (Ctrl iPSCs clone 26). Genomic DNA from the ALT-positive cell lines U2-OS and Saos-2 was set as positive control.
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    (A) Immunofluorescence of DKC1[A353V] fibroblasts (Fib p5) and iPSCs clones 1, 2, 3, and 3-tf at indicated passages (p). PML bodies were stained with an anti-PML antibody (red), and telomeres were labeled with FITC-conjugated PNA probe (green). Interphase nuclei were counterstained with DAPI (blue). Colocalization of PML bodies and telomeres was detected only in <t>VA13</t> cells (positive control). (B) Dot blot of C-circle assay performed on genomic DNA from DKC1[A353V] fibroblasts (Fib p6) and iPSCs clones 1, 1-tf, 2, and 3 at indicated passages (p), as well as iPSCs from control (Ctrl iPSCs clone 26). Genomic DNA from the ALT-positive cell lines U2-OS and Saos-2 was set as positive control.
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    Image Search Results


    (A) Immunofluorescence of DKC1[A353V] fibroblasts (Fib p5) and iPSCs clones 1, 2, 3, and 3-tf at indicated passages (p). PML bodies were stained with an anti-PML antibody (red), and telomeres were labeled with FITC-conjugated PNA probe (green). Interphase nuclei were counterstained with DAPI (blue). Colocalization of PML bodies and telomeres was detected only in VA13 cells (positive control). (B) Dot blot of C-circle assay performed on genomic DNA from DKC1[A353V] fibroblasts (Fib p6) and iPSCs clones 1, 1-tf, 2, and 3 at indicated passages (p), as well as iPSCs from control (Ctrl iPSCs clone 26). Genomic DNA from the ALT-positive cell lines U2-OS and Saos-2 was set as positive control.

    Journal: Stem cell research

    Article Title: Telomere dynamics and hematopoietic differentiation of human DKC1 -mutant induced pluripotent stem cells

    doi: 10.1016/j.scr.2019.101540

    Figure Lengend Snippet: (A) Immunofluorescence of DKC1[A353V] fibroblasts (Fib p5) and iPSCs clones 1, 2, 3, and 3-tf at indicated passages (p). PML bodies were stained with an anti-PML antibody (red), and telomeres were labeled with FITC-conjugated PNA probe (green). Interphase nuclei were counterstained with DAPI (blue). Colocalization of PML bodies and telomeres was detected only in VA13 cells (positive control). (B) Dot blot of C-circle assay performed on genomic DNA from DKC1[A353V] fibroblasts (Fib p6) and iPSCs clones 1, 1-tf, 2, and 3 at indicated passages (p), as well as iPSCs from control (Ctrl iPSCs clone 26). Genomic DNA from the ALT-positive cell lines U2-OS and Saos-2 was set as positive control.

    Article Snippet: Telomerase-deficient WI 38 VA13 subline 2RA cells (VA13, from ATCC) were used as the positive control.

    Techniques: Immunofluorescence, Clone Assay, Staining, Labeling, Positive Control, Dot Blot, Control